High quality Pancreatin 8049-47-6 with best price CAS NO.8049-47-6

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English name: Pancreatin； Pancreatin from hog pancreas
Other names: trypsin; Pancreatic enzyme; Pancreatin; Pancreatic juice extract; Pancreatic digestive hormone; Pancreatic enzyme
CAS number: 8049-47-6
Level: BR
Molecular weight: approximately 23400
Potency: 1:4000
Trypsin activity: ≥ 4000u/g
Pancreatic amylase activity: ≥ 30000u/g
Pancreatic lipase activity: ≥ 7000u/g
Dry weight loss: ≤ 5%
Fat: ≤ 20mg/g
Dissolve method 1: Dissolve trypsin in 90ML of calcium and magnesium free PBS, and then add the PBS to 100ML (the enzyme is difficult to dissolve, and if necessary, it can be dissolved overnight at 4 degrees Celsius). Adjust the pH to around 7.2 and adjust it with 5.6% CaCO3
Dissolving method 2: Maintain the temperature of the solution in the reaction pot at 80 ℃ for at least half an hour, then introduce tap water from the interlayer to lower the temperature of the solution in the pot to 50 ℃. Add the required amount of trypsin at a 1:30 enzyme/substrate ratio, and start the enzymatic hydrolysis reaction under continuous stirring with a stirrer. During this period, adjust the amount of steam introduced into the interlayer of the reaction pot to maintain the temperature at 50 ℃. During the enzymatic hydrolysis process, in order to ensure the maximum biological activity of trypsin and maintain the pH of the reaction solution at 8.0, it is necessary to continuously add dilute NaOH solution to the reaction solution. The enzymatic hydrolysis reaction ends 6 hours after the addition of trypsin
Dissolving method 3: Accurately weigh 8.00g of NaCl, KCl 0.40g、Na2HPO4·7H2O 0.09g、KH2PO4 0.06g、 Phenol Red 0.01g NaHCO3 0.35g， Dissolve in approximately 800mL of distilled water, make up to 1000mL after sufficient dissolution, divide and sterilize for later use, and prepare D-Hanks solution. Accurately weigh 0.125g of trypsin, dissolve it in 100mL of D-Hanks solution, overnight at 4 ℃, filter and pack, and store at -20 ℃ for future use
Appearance: Off white or light yellow amorphous powder. It is a mixture of protease, lipase, and amylase extracted from the pancreas of various animals. Has hygroscopicity. Insoluble in ethanol and ether. The enzyme activity is lost when the aqueous solution boils or comes into contact with acid. Its maximum vitality is in neutral or slightly alkaline media. Mineral acid or excessive hydroxide or carbonate to inactivate it
Purpose: Biochemical research. In neutral or weakly alkaline environments (pH 6.8-8.5), trypsin can convert proteins into peptones, amylase can convert starch into sugars, and lipase can break down fats into glycerol and fatty acids
Storage: 2-8 ℃

Nanjing Duly Biotech Co.,Ltd 

has been established for 11 years. It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.

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