T7 RNA Polymerase 9014-24-8 CAS NO.9014-24-8

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Grouping	enzyme preparation
Keywords	α-Glucosidase/Maltase
Brand	NJDULY
Model	E0090
Place of Origin	Nanjing, China (Mainland)
Payment	T/T
Port	Shanghai
Supply Capacity	100 Kilogram/Kilograms per Month
Packing
Other packaging	Please contact customer service
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English name: T7 RNA Polymerase

This enzyme is derived from Escherichia coli and expressed by the bacteriophilic T7 RNA polymerase gene. It is a highly specific DNA dependent 5 "→ 3" RNA polymerase that recognizes the T7 promoter sequence. T7 RNA polymerase can catalyze the incorporation of NTP downstream of single or double stranded DNA T7 promoter, synthesizing RNA complementary to template DNA downstream of T7 promoter.

Features: T7 RNA polymerase can recognize modified NTP, such as biotinylated, digoxin labeled, and fluorescein labeled NTP, and can be used for the synthesis of various labeled RNAs. At the same time, it has high specificity for the T7 promoter.

Activity definition: The amount of enzyme required to catalyze the incorporation of 1 nmol AMP into a polynucleotide at 37 ℃ for 60 minutes is defined as 1 activity unit.

Activity detection conditions: 40mM Tris HCl (pH 8.0), 6mM MgCl2, 10mM DTT, 2mM spermidine, 0.5mM NTP, 0.6MBq/ml [3H] - ATP, 20 μ g/ml plasmid DNA containing the specific T7 RNA polymerase promoter sequence.

Purity: Free of DNA endonucleases and exonucleases, and free of RNAses.

Enzyme storage solution: 50mM Tris HCl (pH 8.0), 150mM NaCl, 5mM DTT, 0.1mg/ml BSA, 0.5mM Elugent, 50% glycerol.
Translation Buffer (5X): 200mM Tris HCl (pH 7.9 at 25 ℃), 30mM MgCl2, 50mM DTT, 50mM NaCl, 10mM spermidine.

Inactivation or inhibition: Heating at 70 ℃ for 10 minutes can inactivate T7 RNA polymerase. Adding an appropriate amount of EDTA can also inactivate T7 RNA polymerase. Chelating agents, sodium, potassium, or ammonium salts with concentrations greater than 150mM can significantly inhibit the activity of T7 RNA polymerase.

Usage: Used for RNA synthesis, the synthesized RNA can be used as a hybridization probe, genomic DNA sequence analysis, RNase protection assay, antisense RNA synthesis, as an in vitro translation RNA template, substrate for RNA splicing research, RNA secondary structure and RNA protein interaction, nucleic acid amplification analysis, siRNA, miRNA and other small RNAs.

Storage: -20 ℃

 

Nanjing Duly Biotech Co.,Ltd 

has been established for 11 years. It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.

 

Our company’s aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.

FAQ

Q1: Are you a manufacturer?

Answer: Yes, we are factory founded on 2009.

Q2:How to contact with us?

Answer: Search our company name as Nanjing Duly Biotech Co., Ltd. Please contact sales for specific information.

Q3:Can you give me a discount price?

Answer:Surely,It depend on your qty.

Q4: How can i get a sample?

Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.

Q5:What are the main categories of products sold by the company?

Answer: It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.

Q6:Is the company mainly engaged in small packaging or large packaging?

Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.