Enteropeptidase CAS NO.9014-74-8

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Product description: Enterokinase (light chain), CAS number: 9014-74-8, is a highly specific protease that recognizes Asp Asp Asp Asp Lys sequences and hydrolyzes peptides at the C-terminus of Lys. It can convert trypsinogen into trypsin or cleave fusion proteins with this recognition sequence. Due to its high specificity and hydrolysis efficiency, enterokinase is widely used in the development of genetic engineering products. One of its applications is as a tool protease for specific cleavage of recombinant fusion proteins, especially suitable for the biotechnology pharmaceutical industry and research in genetic engineering, biochemistry, molecular biology, and other fields.
This product is a high-purity and highly active bovine enterokinase secreted and expressed by recombinant yeast. It has a wide range of applications (4-45oC, pH 4.5-9.5) and still has partial activity in the presence of various detergents and denaturing agents.

English name of enterokinase: Enterokinase； Enteropeptidase
Other names of enterokinase: enteropeptone enzyme; Intestinal protease; Intestinal peptidase; Intestinal activating enzyme; The right image of EK enzyme shows intestinal digestion
Level: Recombinant
Definition of enterokinase activity: The amount of enzyme required to 95% degrade 0.5 mg of Thioredoxin-NP-27 to NP-27 within 16 hours at 37 ° C is defined as one active unit
Characteristics: Sterilized liquid, recombinant form
Main function: Used for biochemical research. Intestinal kinase is a serine protease that efficiently and specifically hydrolyzes fusion proteins expressed by engineered bacteria (recognition sequence is - (Asp) 4Lys ↓ -) to release the target protein. Due to its high specificity and hydrolysis efficiency, enterokinase is widely used in the development of genetic engineering products; It can serve as a protease to specifically recognize and cleave substrates containing enterokinase cleavage sites. One of its applications is as a tool protease for specific cleavage of recombinant fusion proteins, particularly suitable for research in biotechnology, pharmaceuticals, genetic engineering, biochemistry, molecular biology, and other fields. However, the natural sources of enterokinase are limited, and enterokinase extracted from animal tissues is contaminated with other proteases, which poses difficulties for practical applications. This requires the use of genetic engineering methods to produce high-purity enterokinase. The high-purity enterokinase activity produced by genetic engineering methods is similar to natural enzymes, but the cleavage rate of enterokinase is faster.
Save environment:- 20℃
There are many types of enterokinase chemical reagents, and the classification and grading standards for chemical reagents vary among countries around the world.
IUPAC classifies chemical standard substances as follows:
Grade A: Atomic weight standard.
Grade B: The reference substance closest to Grade A.
Grade C: Standard reagent with a content of 100+-0.02%
Grade D: Standard reagent with a content of 100+-0.05%
Grade E: A reagent with purity determined by comparing with Grade C or Grade D standards
Chemical reagents can be divided into standard reagents, general reagents, biochemical reagents, etc. according to their applications. In China, it is customary to refer to reagents equivalent to IUPAC's C-grade and D-grade as standard reagents

Common factors affecting the activity of recombinant enterokinase:

>2M urea，> 250mM NaCl，> 20mM b-ME，> 0.1% SDS，> 50mM Imidazole.
If the sample solution contains one or more of the above components, in order to obtain ideal enzyme digestion results, please dialyze the sample into 1 x reaction buffer before conducting the enzyme digestion experiment.
·Recombinant bovine enterokinase cleavage reaction:
Each reaction contains 15 μ g of Thioredoxin-NP-27 and varying amounts of enterokinase. After 16 hours of reaction at 37 ° C, the enzyme digestion effect was detected using SDS-PAGE gel at 15 ° C. The enzyme amounts contained in each reaction are:
Lane A: No rEK Lane                 B: 0.0008 units
Lane C: 0.0015 units Lane        D: 0.003 units
Lane E: 0.008 units Lane          F: 0.015 units Lane    G: 0.03 units

Nanjing Duly Biotech Co.,Ltd 

has been established for 11 years. It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.

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Answer: Yes, we are factory founded on 2009.

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Answer: It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.

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