Rnase T1 9026-12-4 CAS NO.9026-12-4

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English name: Ribonulease T1 from Aspergillus oryzae； Rnase T1
CAS number: 9026-12-4

Level: BR
Molecular weight: 11.2kDa, monomer
Source: Escherichia coli cells containing the cloned gene rntA derived from Aspergillus oryzae
Concentration: 1000U/ul
Vitality definition: One active unit refers to the amount of enzyme required to dissolve and hydrolyze yeast RNA at 37 ℃ and pH 7.5 for 15 minutes, resulting in an increase of 1.0 in its 260nm wavelength absorbance value
Enzyme activity analysis mixture: 50mM Tris HCL (pH 7.5), 2mM EDTA, 3mg/ml yeast RNA
Preservation buffer components: 50mM Tris HCL (pH 7.4) and 50% (v/v) glycerol
Quality control: Relevant tests have shown that there is no contamination of endonuclease and exonuclease deoxyribonucleases or proteases. The functional testing method is RNA degradation during plasmid DNA purification (synergistic effect with RNase A)
Inhibitors: Metal ions Mg2+, Ca2+, Zn2+, Fe2+, Cu2+(MgCL2, inhibition efficiency of about 40% at a concentration of 100mM); At a concentration of 10mM, the inhibition efficiency of CaCL2 is about 30%; Zn2+, Fe2+, and Cu2+have strong inhibitory effects at 1mM), single nucleotide (2-GMP, 3-GMP, etc.), guanyl-2-5-guanosine
Inactivation: Thermal inactivation is reversible, and it is recommended to use column purification or phenol/chloroform extraction to remove the enzyme
Characteristic: Ribonuclease T1 is an endonuclease that specifically degrades single stranded RNA at the G residue site. This enzyme cleaves the phosphodiester bond between the 3 '- guanosine residue and the adjacent nucleoside 5' - OH group by forming a nucleoside 2 ', 3' - cyclic phosphate intermediate. The reaction product is a 3 '- GMP terminal oligonucleotide. RNase T1 exhibits activity without the involvement of metal ions
Purpose: Biochemical research. Remove RNA from DNA extract; RNA sequencing; Analysis of ribonuclease protection and synergistic effect with RNase A; Remove RNA from recombinant protein extract; Detecting RNA transcript levels of G-less cassette DNA template in vitro transcription synthesis
Storage: -20 ℃

Nanjing Duly Biotech Co.,Ltd 

has been established for 11 years. It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.

Our company’s aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.

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Answer: Yes, we are factory founded on 2009.

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Q5:What are the main categories of products sold by the company?

Answer: It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.

Q6:Is the company mainly engaged in small packaging or large packaging?

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