High quality M-MLV Reverse transcriptase 9068-38-6 with best price CAS NO.9068-38-6

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Product Name: MMLV Reverse Transcriptase (RNase H -)
Product concentration: 200U/μ l
Storage conditions: stored at -20 ℃, with a shelf life of two years.
Product Description: Reverse transcriptase can start synthesizing complementary DNA strands using RNA or single stranded DNA as templates. MMLV Reverse Transcriptase (RNase H -) is a multi-point mutant type of M-MLV reverse transcriptase. Point mutations remove the active center of RNase H, reducing its activity, decreasing RNA degradation in reverse transcription reactions, and improving the enzyme's heat tolerance. When reverse transcribed at 50 ℃, it maintains 100% activity; It is easier to open the advanced structure of the template, which increases the yield of the first strand of cDNA and makes it easier to obtain full-length cDNA.
Source: E.coli strain, containing the mutated Moroni murine leukemia virus (M-MLV) reverse transcriptase gene.
Activity definition: Using Poly (A) as a template and Oligo (dT) as a primer, the enzyme amount required to catalyze the addition of 1nmol dTTP within 10 minutes at 37 ℃ is defined as one activity unit (U).
Product application: Synthesis of first strand cDNA; RT-PCR reaction.
Storage buffer: 20 mM Tris HCl (pH 7.5), 200 mM NaCl, 0.25 mM EDTA, 0.01% NP-40 (v/v), 2.5 mM DTT, 50% glycerol (v/v).
Product purity: The 200U enzyme and 1 μ g of 16S and 23S rRNA were incubated at 37 ℃ for 1 hour, and there were no visible changes in the electrophoresis bands.
Reaction buffer: [5 × RT Buffer] 250mM Tris HCl (pH 8.3), 15mM MgCl2, 2375 mM KCl, 50mM DTT.
Recommended reaction conditions:

Nanjing Duly Biotech Co.,Ltd 

has been established for 11 years. It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.

Our company’s aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.

FAQ

Q1: Are you a manufacturer?

Answer: Yes, we are factory founded on 2009.

Q2:How to contact with us?

Answer: Search our company name as Nanjing Duly Biotech Co., Ltd. Please contact sales for specific information.

Q3:Can you give me a discount price?

Answer:Surely,It depend on your qty.

Q4: How can i get a sample?

Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.

Q5:What are the main categories of products sold by the company?

Answer: It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.

Q6:Is the company mainly engaged in small packaging or large packaging?

Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.